![]() While SC-islets are less functional when first differentiated in vitro compared to isolated cadaveric islets, transplantation into mice has been shown to increase their maturation. Insulin-producing stem cell-derived islets (SC-islets) provide a virtually unlimited cell source for diabetes cell replacement therapy. See Figure S5 for additional immunofluorescent staining of hESC-derived grafts. All data are presented as mean ± SEM plus individual biological replicates (n = 3 animals/group). (D) Area of synaptophysin or trypsin immunoreactivity relative to the total graft area and synaptophysin+ area relative to trypsin+ area for each graft. (C) Area of insulin (ins) or glucagon (gcg) immunoreactivity relative to total synaptophysin immunoreactivity and insulin+ area relative to glucagon+ area for each graft. DAPI nuclear staining is shown in gray in all images. Shown are (A) insulin (red) and glucagon (green) and (B) synaptophysin (red, endocrine marker) and trypsin (green, exocrine marker). ![]() Grafts from Rats Contained a Higher Proportion of Insulin:Glucagon and Synaptophysin:Trypsin Immunoreactivity Compared with Grafts from Mice (A and B) Representative immunofluorescence images of whole hESC-derived grafts (graft and kidney tissue is delineated by a dashed white line scale bars, 500 mm) and higher-magnification insets (scale bars, 100 mm) at 22 weeks post-transplantation. See Figure S4 for the human C-peptide stimulation index following meal challenges. Data are presented as mean ± SEM plus individual biological replicates in bar graphs. The black dashed line indicates the lower limit of detection for each analyte. (F) Plasma human insulin, glucagon, and GLP-1 levels at 22 or 33 weeks post-transplantation (n = 10 animals/group). ![]() For area under the curve, *p <</a> 0.05, ***p < 0.001 two-tailed t test.
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